NF-KB p65 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16533-50, BZ16533-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: Q04206

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ICC/IF, IP, , FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 60 kDa; Observed MW: 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of NF-KB p65 in HT-1080 using NFκB p65 antibody., Western blot analysis of NF-KB p65 in MCF-7, Hela, Molt4, Raji and Jurkat lysates using NF-KB p65 antibody., Western blot analysis of NFκB p65 expression in HeLa lysates using NF-KB p65R antibody, Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder using NFκB p65 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Immunogen: A synthesized peptide derived from human NF-κB p65

Conjugate: Unconjugated

Modification: Unmodified